Hotspot

petN-psbM

LSC · 29690-30914 · 1225 bp

Back to Calystegia

Pi 0.00381

MarkerSeek score 45.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1225
variable_sites: 7
pi: 0.00381
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TTCCTACCGCCTTTCTCCTT	50.0	50.0	58.2	58.3	1364 bp	1.0	76.4
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TCCTACCGCCTTTCTCCTTA	50.0	50.0	58.2	57.5	1363 bp	1.0	74.9
petN-psbM_p3	TAGTGTGGGGAAGAAGTGGA	TTCCTACCGCCTTTCTCCTT	50.0	50.0	57.6	58.3	1307 bp	1.0	74.4
petN-psbM_p4	GTAGTGTGGGGAAGAAGTGG	TTCCTACCGCCTTTCTCCTT	55.0	50.0	57.5	58.3	1308 bp	1.0	73.9
petN-psbM_p5	TAGTGTGGGGAAGAAGTGGA	TCCTACCGCCTTTCTCCTTA	50.0	50.0	57.6	57.5	1306 bp	1.0	72.9