Hotspot

atpF-atpH

LSC · 13161-13521 · 361 bp

Back to Calystegia

Pi 0.00185

MarkerSeek score 55.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 361
variable_sites: 1
pi: 0.00185
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TTAAACCCGAAACTCCCAGC	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.1	58.0	472-473 bp	1.0	79.5
atpF-atpH_p2	TTAAACCCGAAACTCCCAGC	TTGGTTGTGGCATTAGCACT	50.0	45.0	58.1	58.0	473-474 bp	1.0	79.5
atpF-atpH_p3	CAGTGGCCCAAAGAAAGGAA	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.3	58.0	445-446 bp	1.0	77.9
atpF-atpH_p4	CAGTGGCCCAAAGAAAGGAA	TTGGTTGTGGCATTAGCACT	50.0	45.0	58.3	58.0	446-447 bp	1.0	77.9
atpF-atpH_p5	AGTGGCCCAAAGAAAGGAAA	TGGTTGTGGCATTAGCACTT	45.0	45.0	57.5	58.0	444-445 bp	1.0	76.6