Hotspot

ycf2

Genome · 32573-39517 · 6945 bp

Back to Calyptothecium

Pi 0.00288

MarkerSeek score 54.20

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6945
variable_sites: 20
pi: 0.00288
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TAATAGATTCTTCACGTGGAA	TGCTGAATGCTTTTGTCAGT	33.3	40.0	52.0	56.2	250 bp	1.0	41.0
ycf2_p2	TTAATAGATTCTTCACGTGGA	TGCTGAATGCTTTTGTCAGT	33.3	40.0	52.0	56.2	251 bp	1.0	41.0
ycf2_p3	TTCACGTGGAAGATATTTAGA	TGCTGAATGCTTTTGTCAGT	33.3	40.0	52.0	56.2	240 bp	1.0	41.0
ycf2_p4	TTAATAGATTCTTCACGTGGAA	TGCTGAATGCTTTTGTCAGT	31.8	40.0	53.0	56.2	251 bp	1.0	41.0
ycf2_p5	TTTAATAGATTCTTCACGTGGA	TGCTGAATGCTTTTGTCAGT	31.8	40.0	53.0	56.2	252 bp	1.0	41.0