Hotspot

trnF-GAA

Genome · 54162-54234 · 73 bp

Back to Calyptothecium

Pi 0.0137

MarkerSeek score 60.40

Primer available yes

Label trnF-GAA

Feature Metadata

label_name: trnF-GAA
feature_type: tRNA
region: Genome
length_bp: 73
variable_sites: 1
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA_p1	TGCAGAGACTCAATGGAAGC	GGGCGATTATCTGGTTGGTT	50.0	50.0	57.9	57.9	978 bp	1.0	78.8
trnF-GAA_p2	GGGTATGGCGGAATTGGTAG	GGGCGATTATCTGGTTGGTT	55.0	50.0	58.1	57.9	1134 bp	1.0	78.8
trnF-GAA_p3	AAGTCCCTCTACCCCCAAAA	GGGCGATTATCTGGTTGGTT	50.0	50.0	57.9	57.9	803 bp	1.0	78.5
trnF-GAA_p4	TGCAGAGACTCAATGGAAGC	AGTTGGATTGGTTGGCCTTT	50.0	45.0	57.9	57.9	579 bp	1.0	78.5
trnF-GAA_p5	GGAATTGGTAGACGCTACGG	GGGCGATTATCTGGTTGGTT	55.0	50.0	58.2	57.9	1125 bp	1.0	78.4