Hotspot

ndhG

Genome · 102712-103314 · 603 bp

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Pi 0.00498

MarkerSeek score 61.60

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: Genome
length_bp: 603
variable_sites: 3
pi: 0.00498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ACATCCATCCCAAGGTACCA	GCAGCACGGTATATTGGTCA	50.0	50.0	58.0	58.1	1793-1795 bp	1.0	79.7
ndhG_p2	ACATCCATCCCAAGGTACCA	GCTTCCGTGGACGTATTCAT	50.0	50.0	58.0	58.1	1690-1692 bp	1.0	79.7
ndhG_p3	TCGCACCATATTCCGACTTG	GCAGCACGGTATATTGGTCA	50.0	50.0	58.1	58.1	1320-1321 bp	1.0	79.3
ndhG_p4	ACATCCATCCCAAGGTACCA	TTGCCTGCGAAGTATGTGTT	50.0	45.0	58.0	58.1	1651-1653 bp	1.0	79.3
ndhG_p5	TCGCACCATATTCCGACTTG	GCTTCCGTGGACGTATTCAT	50.0	50.0	58.1	58.1	1217-1218 bp	1.0	79.2