Hotspot

ndhE

Genome · 102379-102681 · 303 bp

Back to Calyptothecium

Pi 0.0033

MarkerSeek score 42.80

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: Genome
length_bp: 303
variable_sites: 1
pi: 0.0033
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	ACATCCATCCCAAGGTACCA	CCCTCTTGGACTATAGGCGA	50.0	55.0	58.0	58.0	911-912 bp	1.0	80.3
ndhE_p2	ACATCCATCCCAAGGTACCA	GTAGCTGCCGCACAAATTTT	50.0	45.0	58.0	57.9	1010-1011 bp	1.0	79.7
ndhE_p3	ACATCCATCCCAAGGTACCA	TGGCCCTCTTGGACTATAGG	50.0	55.0	58.0	57.9	914-915 bp	1.0	79.5
ndhE_p4	ACATCTGTAGGACACGCTCT	CCCTCTTGGACTATAGGCGA	50.0	55.0	58.2	58.0	883-884 bp	1.0	79.2
ndhE_p5	ACATCCATCCCAAGGTACCA	AGCCCGTCGAGAAAATTTGA	50.0	45.0	58.0	57.8	663-664 bp	1.0	79.0