Hotspot

ndhA

Genome · 104051-105897 · 1847 bp

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Pi 0.00271

MarkerSeek score 59.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: Genome
length_bp: 1847
variable_sites: 5
pi: 0.00271
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AGTTTTGAAGCCTATTTAGCA	TGGGAGAGGTTGATCGTTAA	33.3	45.0	53.4	55.9	1980 bp	1.0	40.2
ndhA_p2	AGTTTTGAAGCCTATTTAGCA	TGGGAGAGGTTGATCGTTAAA	33.3	42.9	53.4	56.7	1980 bp	1.0	40.2
ndhA_p3	AGTTTTGAAGCCTATTTAGCA	ATGGGAGAGGTTGATCGTTA	33.3	45.0	53.4	55.6	1981 bp	1.0	40.2
ndhA_p4	AGTTTTGAAGCCTATTTAGCA	TATGGGAGAGGTTGATCGTT	33.3	45.0	53.4	55.6	1982 bp	1.0	40.2
ndhA_p5	AGTTTTGAAGCCTATTTAGCA	TTATGGGAGAGGTTGATCGT	33.3	45.0	53.4	55.6	1983 bp	1.0	40.2