Hotspot

rpl23-trnI-CAU

Genome · 86777-86979 · 203 bp

Back to Calyptothecium

Pi 0.00495
MarkerSeek score 44.90
Primer available yes
Label rpl23-trnI-CAU

Feature Metadata

label_name
rpl23-trnI-CAU
feature_type
igs
region
Genome
length_bp
203
variable_sites
1
pi
0.00495
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnI-CAU_p1 AGTCGATTTTTGATTAACATCA TGGAGAATTCGCAGGTTCAA 27.3 45.0 52.1 57.4 330-331 bp 1.0 40.5
rpl23-trnI-CAU_p2 AGTCGATTTTTGATTAACATCA TTGGAGAATTCGCAGGTTCA 27.3 45.0 52.1 57.4 331-332 bp 1.0 40.5
rpl23-trnI-CAU_p3 AGTCGATTTTTGATTAACATCA AATTCGCAGGTTCAACTCCT 27.3 45.0 52.1 57.4 325-326 bp 1.0 40.5
rpl23-trnI-CAU_p4 AGTCGATTTTTGATTAACATCAA TGGAGAATTCGCAGGTTCAA 26.1 45.0 53.0 57.4 330-331 bp 1.0 40.5
rpl23-trnI-CAU_p5 AGTCGATTTTTGATTAACATCAA TTGGAGAATTCGCAGGTTCA 26.1 45.0 53.0 57.4 331-332 bp 1.0 40.5