Hotspot

trnT-GGU-psbD

Genome · 40605-41268 · 664 bp

Back to Calyptothecium

Pi 0.00151

MarkerSeek score 59.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: Genome
length_bp: 664
variable_sites: 1
pi: 0.00151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	TCTTCGTAGCCAGTCATCCA	55.0	50.0	58.1	58.2	797-806 bp	1.0	78.4
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	TCTTCGTAGCCAGTCATCCA	50.0	50.0	58.1	58.2	800-809 bp	1.0	78.2
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	TCTTCGTAGCCAGTCATCCA	50.0	50.0	58.3	58.2	774-783 bp	1.0	76.6
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	CGATCTCTTCGTAGCCAGTC	55.0	55.0	58.1	57.6	802-811 bp	1.0	76.4
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	CGATCTCTTCGTAGCCAGTC	50.0	55.0	58.1	57.6	805-814 bp	1.0	76.3