Hotspot

chlB-trnK-UUU

Genome · 27604-28341 · 738 bp

Back to Calyptothecium

Pi 0.00271

MarkerSeek score 60.30

Primer available yes

Label chlB-trnK-UUU

Feature Metadata

label_name: chlB-trnK-UUU
feature_type: igs
region: Genome
length_bp: 738
variable_sites: 2
pi: 0.00271
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB-trnK-UUU_p1	TGAAAAATTCGCGCGTCAAA	AGTCGCACTTAAAAGCCGAG	40.0	50.0	57.9	58.6	865-866 bp	1.0	74.8
chlB-trnK-UUU_p2	TGAAAAATTCGCGCGTCAAA	TAGTCGCACTTAAAAGCCGA	40.0	45.0	57.9	57.3	866-867 bp	1.0	73.5
chlB-trnK-UUU_p3	TGAAAAATTCGCGCGTCAAA	TCGCACTTAAAAGCCGAGTA	40.0	45.0	57.9	57.3	863-864 bp	1.0	73.5
chlB-trnK-UUU_p4	TGAAAAATTCGCGCGTCAAA	CGCACTTAAAAGCCGAGTAC	40.0	50.0	57.9	57.2	862-863 bp	1.0	73.1
chlB-trnK-UUU_p5	CTGAAAAATTCGCGCGTCAA	AGTCGCACTTAAAAGCCGAG	45.0	50.0	58.3	58.6	866-867 bp	1.0	73.0