primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
chlB-trnK-UUU_p1	chlB-trnK-UUU	1	TGAAAAATTCGCGCGTCAAA	AGTCGCACTTAAAAGCCGAG	20	20	40.0	50.0	57.9	58.6	31.0	6.4	0.677287	27604	28341	865-866	1.000000	74.8
chlB-trnK-UUU_p2	chlB-trnK-UUU	2	TGAAAAATTCGCGCGTCAAA	TAGTCGCACTTAAAAGCCGA	20	20	40.0	45.0	57.9	57.3	31.0	3.0	0.835439	27604	28341	866-867	1.000000	73.5
chlB-trnK-UUU_p3	chlB-trnK-UUU	3	TGAAAAATTCGCGCGTCAAA	TCGCACTTAAAAGCCGAGTA	20	20	40.0	45.0	57.9	57.3	31.0	3.0	0.835439	27604	28341	863-864	1.000000	73.5
chlB-trnK-UUU_p4	chlB-trnK-UUU	4	TGAAAAATTCGCGCGTCAAA	CGCACTTAAAAGCCGAGTAC	20	20	40.0	50.0	57.9	57.2	31.0	0.0	0.888947	27604	28341	862-863	1.000000	73.1
chlB-trnK-UUU_p5	chlB-trnK-UUU	5	CTGAAAAATTCGCGCGTCAA	AGTCGCACTTAAAAGCCGAG	20	20	45.0	50.0	58.3	58.6	31.0	6.4	0.901354	27604	28341	866-867	1.000000	73.0
ycf2_p1	ycf2	1	TAATAGATTCTTCACGTGGAA	TGCTGAATGCTTTTGTCAGT	21	20	33.3	40.0	52.0	56.2	17.0	0.0	8.806068	36839	36958	250-250	1.000000	41.0
ycf2_p2	ycf2	2	TTAATAGATTCTTCACGTGGA	TGCTGAATGCTTTTGTCAGT	21	20	33.3	40.0	52.0	56.2	17.0	0.0	8.806068	36839	36958	251-251	1.000000	41.0
ycf2_p3	ycf2	3	TTCACGTGGAAGATATTTAGA	TGCTGAATGCTTTTGTCAGT	21	20	33.3	40.0	52.0	56.2	17.0	0.0	8.806068	36839	36958	240-240	1.000000	41.0
ycf2_p4	ycf2	4	TTAATAGATTCTTCACGTGGAA	TGCTGAATGCTTTTGTCAGT	22	20	31.8	40.0	53.0	56.2	17.0	0.0	8.885388	36839	36958	251-251	1.000000	41.0
ycf2_p5	ycf2	5	TTTAATAGATTCTTCACGTGGA	TGCTGAATGCTTTTGTCAGT	22	20	31.8	40.0	53.0	56.2	17.0	0.0	8.885388	36839	36958	252-252	1.000000	41.0
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	TCTTCGTAGCCAGTCATCCA	20	20	55.0	50.0	58.1	58.2	7.6	0.0	0.216739	40605	41268	797-806	1.000000	78.4
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	TCTTCGTAGCCAGTCATCCA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.238894	40605	41268	800-809	1.000000	78.2
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GGCGTAAGTCATCGGTTCAA	TCTTCGTAGCCAGTCATCCA	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.435478	40605	41268	774-783	1.000000	76.6
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	CGATCTCTTCGTAGCCAGTC	20	20	55.0	55.0	58.1	57.6	7.6	0.0	0.457000	40605	41268	802-811	1.000000	76.4
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TCAGTGGTAGAGTAACGCCA	CGATCTCTTCGTAGCCAGTC	20	20	50.0	55.0	58.1	57.6	0.0	0.0	0.479155	40605	41268	805-814	1.000000	76.3
trnF-GAA_p1	trnF-GAA	1	TGCAGAGACTCAATGGAAGC	GGGCGATTATCTGGTTGGTT	20	20	50.0	50.0	57.9	57.9	5.0	0.0	0.157788	54162	54234	978-978	1.000000	78.8
trnF-GAA_p2	trnF-GAA	2	GGGTATGGCGGAATTGGTAG	GGGCGATTATCTGGTTGGTT	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.160573	54162	54234	1134-1134	1.000000	78.8
trnF-GAA_p3	trnF-GAA	3	AAGTCCCTCTACCCCCAAAA	GGGCGATTATCTGGTTGGTT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.196013	54162	54234	803-803	1.000000	78.5
trnF-GAA_p4	trnF-GAA	4	TGCAGAGACTCAATGGAAGC	AGTTGGATTGGTTGGCCTTT	20	20	50.0	45.0	57.9	57.9	5.0	3.3	0.206646	54162	54234	579-579	1.000000	78.5
trnF-GAA_p5	trnF-GAA	5	GGAATTGGTAGACGCTACGG	GGGCGATTATCTGGTTGGTT	20	20	55.0	50.0	58.2	57.9	9.8	0.0	0.203686	54162	54234	1125-1125	1.000000	78.4
atpE-atpB_p1	atpE-atpB	1	CGGCCACTGTTTGTAGATGA	AGGCTGCAACCTTACAAGTG	20	20	50.0	50.0	57.9	58.4	2.2	1.8	0.484098	57620	57625	124-124	1.000000	76.8
atpE-atpB_p2	atpE-atpB	2	TCGGCCACTGTTTGTAGATG	AGGCTGCAACCTTACAAGTG	20	20	50.0	50.0	57.9	58.4	2.2	1.8	0.484098	57620	57625	125-125	1.000000	76.8
atpE-atpB_p3	atpE-atpB	3	CGGCCACTGTTTGTAGATGA	GCTGCAACCTTACAAGTGGA	20	20	50.0	50.0	57.9	58.4	2.2	0.0	0.488702	57620	57625	122-122	1.000000	76.7
atpE-atpB_p4	atpE-atpB	4	TCGGCCACTGTTTGTAGATG	GCTGCAACCTTACAAGTGGA	20	20	50.0	50.0	57.9	58.4	2.2	0.0	0.488702	57620	57625	123-123	1.000000	76.7
atpE-atpB_p5	atpE-atpB	5	CGGCCACTGTTTGTAGATGA	TGAAGCTACTGCAAAGGCTG	20	20	50.0	50.0	57.9	58.5	2.2	7.6	0.566272	57620	57625	138-138	1.000000	76.0
rbcL_p1	rbcL	1	TCATAGCAACAGCTCGAACC	CTTCATCCATAGGCTGCCAG	20	20	50.0	55.0	58.0	58.1	0.0	22.7	0.116601	59599	61026	2887-2888	1.000000	79.4
rbcL_p2	rbcL	2	CATAGCAACAGCTCGAACCT	CTTCATCCATAGGCTGCCAG	20	20	50.0	55.0	58.0	58.1	0.0	22.7	0.122191	59599	61026	2886-2887	1.000000	79.4
rbcL_p3	rbcL	3	CACTCATAGCAACAGCTCGA	CTTCATCCATAGGCTGCCAG	20	20	50.0	55.0	57.7	58.1	0.0	22.7	0.390718	59599	61026	2890-2891	1.000000	77.2
rbcL_p4	rbcL	4	CCCACTCATAGCAACAGCTC	CTTCATCCATAGGCTGCCAG	20	20	55.0	55.0	58.3	58.1	0.0	22.7	0.445114	59599	61026	2892-2893	1.000000	76.8
rbcL_p5	rbcL	5	TTGAGTTGTAGGGAGGGACC	CTTCATCCATAGGCTGCCAG	20	20	55.0	55.0	58.3	58.1	0.0	22.7	0.453508	59599	61026	2182-2183	1.000000	76.8
psaI-ycf4_p1	psaI-ycf4	1	TTGAACAAGACGAGATAATCT	GCTCTACCCAAAGCCATTCA	21	20	33.3	50.0	52.3	57.9	0.0	0.0	6.784098	62828	63179	409-421	1.000000	42.3
psaI-ycf4_p2	psaI-ycf4	2	ATTGAACAAGACGAGATAATCT	GCTCTACCCAAAGCCATTCA	22	20	31.8	50.0	53.0	57.9	0.0	0.0	7.103925	62828	63179	410-422	1.000000	42.3
psaI-ycf4_p3	psaI-ycf4	3	TGAACAAGACGAGATAATCTAA	GCTCTACCCAAAGCCATTCA	22	20	31.8	50.0	52.4	57.9	0.0	0.0	7.684609	62828	63179	408-420	1.000000	42.3
psaI-ycf4_p4	psaI-ycf4	4	TTGAACAAGACGAGATAATCTA	GCTCTACCCAAAGCCATTCA	22	20	31.8	50.0	52.4	57.9	0.0	0.0	7.684609	62828	63179	409-421	1.000000	42.3
psaI-ycf4_p5	psaI-ycf4	5	TTGAACAAGACGAGATAATCT	AAAAACCCGAATGCACCAAA	21	20	33.3	40.0	52.3	57.0	0.0	0.0	7.645394	62828	63179	482-494	1.000000	42.0
cemA_p1	cemA	1	TTTCCGTTTTAGCGAATCTT	ACTCTACATTAGTAATCAAAAGAACT	20	26	35.0	26.9	53.5	54.5	0.0	0.0	13.984871	63852	65369	1622-1622	1.000000	40.1
cemA_p2	cemA	2	TTTTCCGTTTTAGCGAATCT	ACTCTACATTAGTAATCAAAAGAACT	20	26	35.0	26.9	53.5	54.5	0.0	0.0	13.984871	63852	65369	1623-1623	1.000000	40.1
cemA_p3	cemA	3	TTTTCCGTTTTAGCGAATCTT	ACTCTACATTAGTAATCAAAAGAACT	21	26	33.3	26.9	54.4	54.5	0.0	0.0	14.108081	63852	65369	1623-1623	1.000000	40.1
cemA_p4	cemA	4	TTTTTCCGTTTTAGCGAATCT	ACTCTACATTAGTAATCAAAAGAACT	21	26	33.3	26.9	54.4	54.5	0.0	0.0	14.108081	63852	65369	1624-1624	1.000000	40.1
cemA_p5	cemA	5	TTTTTCCGTTTTAGCGAATCTT	ACTCTACATTAGTAATCAAAAGAACT	22	26	31.8	26.9	55.2	54.5	0.0	0.0	14.309432	63852	65369	1624-1624	1.000000	40.1
petD-rps11_p1	petD-rps11	1	GCCGTATCCCTTTGGTTAGG	AGCTATTCGTCGGAGTGGTA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.085272	79500	79819	488-489	1.000000	80.6
petD-rps11_p2	petD-rps11	2	CTTTTCGTCGTCCAGTAGCA	AGCTATTCGTCGGAGTGGTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.108770	79500	79819	532-533	1.000000	80.3
petD-rps11_p3	petD-rps11	3	CGTCCAGTAGCAACAACAGT	AGCTATTCGTCGGAGTGGTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.182579	79500	79819	524-525	1.000000	79.8
petD-rps11_p4	petD-rps11	4	GTCGTCCAGTAGCAACAACA	AGCTATTCGTCGGAGTGGTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.187768	79500	79819	526-527	1.000000	79.7
petD-rps11_p5	petD-rps11	5	TCGTCCAGTAGCAACAACAG	AGCTATTCGTCGGAGTGGTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.197360	79500	79819	525-526	1.000000	79.6
rpl16_p1	rpl16	1	CGTACAACTACTGCTCGGAC	GGAGACCGCAAACTTCGTAT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.113503	82175	83437	2034-2034	1.000000	79.3
rpl16_p2	rpl16	2	ACAGGACCAAAAACACGTGT	GGAGACCGCAAACTTCGTAT	20	20	45.0	50.0	58.2	58.0	17.2	0.0	0.176991	82175	83437	2148-2148	1.000000	78.8
rpl16_p3	rpl16	3	TCGTACAACTACTGCTCGGA	GGAGACCGCAAACTTCGTAT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.193735	82175	83437	2035-2035	1.000000	78.6
rpl16_p4	rpl16	4	CCACTGTTGTCGGCTACATT	GGAGACCGCAAACTTCGTAT	20	20	50.0	50.0	58.2	58.0	4.4	0.0	0.197560	82175	83437	1887-1887	1.000000	78.6
rpl16_p5	rpl16	5	GGACCAAAAACACGTGTTCC	GGAGACCGCAAACTTCGTAT	20	20	50.0	50.0	57.8	58.0	32.4	0.0	0.203763	82175	83437	2145-2145	1.000000	78.6
rpl23-trnI-CAU_p1	rpl23-trnI-CAU	1	AGTCGATTTTTGATTAACATCA	TGGAGAATTCGCAGGTTCAA	22	20	27.3	45.0	52.1	57.4	0.0	11.5	8.413422	86777	86979	330-331	1.000000	40.5
rpl23-trnI-CAU_p2	rpl23-trnI-CAU	2	AGTCGATTTTTGATTAACATCA	TTGGAGAATTCGCAGGTTCA	22	20	27.3	45.0	52.1	57.4	0.0	11.5	8.413422	86777	86979	331-332	1.000000	40.5
rpl23-trnI-CAU_p3	rpl23-trnI-CAU	3	AGTCGATTTTTGATTAACATCA	AATTCGCAGGTTCAACTCCT	22	20	27.3	45.0	52.1	57.4	0.0	0.0	8.422347	86777	86979	325-326	1.000000	40.5
rpl23-trnI-CAU_p4	rpl23-trnI-CAU	4	AGTCGATTTTTGATTAACATCAA	TGGAGAATTCGCAGGTTCAA	23	20	26.1	45.0	53.0	57.4	0.0	11.5	8.542600	86777	86979	330-331	1.000000	40.5
rpl23-trnI-CAU_p5	rpl23-trnI-CAU	5	AGTCGATTTTTGATTAACATCAA	TTGGAGAATTCGCAGGTTCA	23	20	26.1	45.0	53.0	57.4	0.0	11.5	8.542600	86777	86979	331-332	1.000000	40.5
ndhE_p1	ndhE	1	ACATCCATCCCAAGGTACCA	CCCTCTTGGACTATAGGCGA	20	20	50.0	55.0	58.0	58.0	15.9	4.4	0.023392	102379	102681	911-912	1.000000	80.3
ndhE_p2	ndhE	2	ACATCCATCCCAAGGTACCA	GTAGCTGCCGCACAAATTTT	20	20	50.0	45.0	58.0	57.9	15.9	2.0	0.084764	102379	102681	1010-1011	1.000000	79.7
ndhE_p3	ndhE	3	ACATCCATCCCAAGGTACCA	TGGCCCTCTTGGACTATAGG	20	20	50.0	55.0	58.0	57.9	15.9	0.0	0.123344	102379	102681	914-915	1.000000	79.5
ndhE_p4	ndhE	4	ACATCTGTAGGACACGCTCT	CCCTCTTGGACTATAGGCGA	20	20	50.0	55.0	58.2	58.0	0.0	4.4	0.162465	102379	102681	883-884	1.000000	79.2
ndhE_p5	ndhE	5	ACATCCATCCCAAGGTACCA	AGCCCGTCGAGAAAATTTGA	20	20	50.0	45.0	58.0	57.8	15.9	0.0	0.199103	102379	102681	663-664	1.000000	79.0
ndhG_p1	ndhG	1	ACATCCATCCCAAGGTACCA	GCAGCACGGTATATTGGTCA	20	20	50.0	50.0	58.0	58.1	15.9	0.0	0.072086	102712	103314	1793-1795	1.000000	79.7
ndhG_p2	ndhG	2	ACATCCATCCCAAGGTACCA	GCTTCCGTGGACGTATTCAT	20	20	50.0	50.0	58.0	58.1	15.9	0.0	0.084258	102712	103314	1690-1692	1.000000	79.7
ndhG_p3	ndhG	3	TCGCACCATATTCCGACTTG	GCAGCACGGTATATTGGTCA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.117307	102712	103314	1320-1321	1.000000	79.3
ndhG_p4	ndhG	4	ACATCCATCCCAAGGTACCA	TTGCCTGCGAAGTATGTGTT	20	20	50.0	45.0	58.0	58.1	15.9	0.0	0.129314	102712	103314	1651-1653	1.000000	79.3
ndhG_p5	ndhG	5	TCGCACCATATTCCGACTTG	GCTTCCGTGGACGTATTCAT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129479	102712	103314	1217-1218	1.000000	79.2
ndhA_p1	ndhA	1	AGTTTTGAAGCCTATTTAGCA	TGGGAGAGGTTGATCGTTAA	21	20	33.3	45.0	53.4	55.9	0.0	0.0	7.726422	104051	105897	1980-1980	1.000000	40.2
ndhA_p2	ndhA	2	AGTTTTGAAGCCTATTTAGCA	TGGGAGAGGTTGATCGTTAAA	21	21	33.3	42.9	53.4	56.7	0.0	0.0	7.941183	104051	105897	1980-1980	1.000000	40.2
ndhA_p3	ndhA	3	AGTTTTGAAGCCTATTTAGCA	ATGGGAGAGGTTGATCGTTA	21	20	33.3	45.0	53.4	55.6	0.0	0.0	7.963248	104051	105897	1981-1981	1.000000	40.2
ndhA_p4	ndhA	4	AGTTTTGAAGCCTATTTAGCA	TATGGGAGAGGTTGATCGTT	21	20	33.3	45.0	53.4	55.6	0.0	0.0	7.963248	104051	105897	1982-1982	1.000000	40.2
ndhA_p5	ndhA	5	AGTTTTGAAGCCTATTTAGCA	TTATGGGAGAGGTTGATCGT	21	20	33.3	45.0	53.4	55.6	0.0	0.0	7.963248	104051	105897	1983-1983	1.000000	40.2
chlL_p1	chlL	1	CCTGTTTCGCATTCAAAAGTGA	AGATTCTTTAATATGACCATAGAACA	22	26	40.9	26.9	58.0	54.3	0.0	0.0	11.714906	114062	114937	1041-1041	1.000000	40.2
chlL_p2	chlL	2	GTGTGGTAATTACCTGTTTCGC	AGATTCTTTAATATGACCATAGAACA	22	26	45.5	26.9	57.9	54.3	30.1	0.0	11.753037	114062	114937	1053-1053	1.000000	40.2
chlL_p3	chlL	3	ACCTGTTTCGCATTCAAAAGT	AGATTCTTTAATATGACCATAGAACA	21	26	38.1	26.9	56.9	54.3	0.0	0.0	11.754092	114062	114937	1042-1042	1.000000	40.2
chlL_p4	chlL	4	TGTGGTAATTACCTGTTTCGCA	AGATTCTTTAATATGACCATAGAACA	22	26	40.9	26.9	58.3	54.3	30.1	0.0	11.937322	114062	114937	1052-1052	1.000000	40.2
chlL_p5	chlL	5	ACCTGTTTCGCATTCAAAAGTG	AGATTCTTTAATATGACCATAGAACA	22	26	40.9	26.9	58.3	54.3	0.0	0.0	11.972967	114062	114937	1042-1042	1.000000	40.2