Hotspot

rpl16

LSC · 81001-82588 · 1588 bp

Back to Calypso

Pi 0.0166

MarkerSeek score 57.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1588
variable_sites: 26
pi: 0.0166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGGGCACTGCTTCTTTGATT	GCAGGGTTCCCTTACAAACA	45.0	50.0	58.0	58.0	2153-2176 bp	1.0	81.7
rpl16_p2	TTGGGCACTGCTTCTTTGAT	GCAGGGTTCCCTTACAAACA	45.0	50.0	58.0	58.0	2154-2177 bp	1.0	81.7
rpl16_p3	CGAGCAATCGCACCAAAAAT	GCAGGGTTCCCTTACAAACA	45.0	50.0	58.0	58.0	2319-2342 bp	1.0	81.4
rpl16_p4	ACGTGCGTACAATAACAGCT	GCAGGGTTCCCTTACAAACA	45.0	50.0	57.9	58.0	2204-2227 bp	1.0	81.0
rpl16_p5	TATCATGCCGTTGTCACGTT	GCAGGGTTCCCTTACAAACA	45.0	50.0	57.9	58.0	2236-2259 bp	1.0	80.9