Hotspot

rpl2-trnH-GUG

IRa · 148685-148727 · 43 bp

Back to Calypso

Pi 0.0233

MarkerSeek score 65.90

Primer available yes

Label rpl2-trnH-GUG

Feature Metadata

label_name: rpl2-trnH-GUG
feature_type: igs
region: IRa
length_bp: 43
variable_sites: 1
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnH-GUG_p1	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.0	58.1	193 bp	1.0	79.2
rpl2-trnH-GUG_p2	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	50.0	50.0	58.0	57.9	179 bp	1.0	79.2
rpl2-trnH-GUG_p3	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	50.0	50.0	58.0	57.9	178 bp	1.0	79.2
rpl2-trnH-GUG_p4	GTTATCCTGCGCTTGGAAGA	CTAGATAAGGGAAGGGCGGA	50.0	55.0	58.0	58.0	209 bp	0.5	59.7
rpl2-trnH-GUG_p5	GTTATCCTGCGCTTGGAAGA	ATAAGGGAAGGGCGGATGTA	50.0	50.0	58.0	57.9	205 bp	0.5	58.9