Hotspot

ndhD-psaC

SSC · 113981-114356 · 376 bp

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Pi 0.0347

MarkerSeek score 62.70

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 376
variable_sites: 13
pi: 0.0347
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CCTGTCAGTAAACTGGGTCC	AGATGTGAATCCGCTTGTCC	55.0	50.0	57.5	58.0	494 bp	1.0	78.2
ndhD-psaC_p2	CCTGTCAGTAAACTGGGTCC	ATGTGAATCCGCTTGTCCAA	55.0	45.0	57.5	57.8	492 bp	1.0	76.9
ndhD-psaC_p3	CCTGTCAGTAAACTGGGTCC	CGCAGCATGGGTCTTTCTTA	55.0	50.0	57.5	58.3	428 bp	1.0	76.7
ndhD-psaC_p4	CCTGTCAGTAAACTGGGTCC	GATGTGAATCCGCTTGTCCA	55.0	50.0	57.5	58.3	493 bp	1.0	76.4
ndhD-psaC_p5	CCTGTCAGTAAACTGGGTCC	CGCTTGTCCAACGGATTTTT	55.0	45.0	57.5	57.6	483 bp	1.0	75.2