Hotspot

rpl20-rps12

LSC · 67891-68667 · 777 bp

Back to Calypso

Pi 0.00964

MarkerSeek score 68.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 777
variable_sites: 7
pi: 0.00964
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TTGTTGAGTAGCCGTTCGAG	GAAATCTCCCGCTCTTCGAG	50.0	55.0	57.9	58.2	898-949 bp	1.0	82.9
rpl20-rps12_p2	TTGTTGAGTAGCCGTTCGAG	TAGAAACGCAAGACAGCCAA	50.0	45.0	57.9	57.8	931-982 bp	1.0	82.1
rpl20-rps12_p3	TTGTTGAGTAGCCGTTCGAG	TTAGAAACGCAAGACAGCCA	50.0	45.0	57.9	57.8	932-983 bp	1.0	82.1
rpl20-rps12_p4	GTCAGTCTTGAATGAGCCCC	GAAATCTCCCGCTCTTCGAG	55.0	55.0	58.3	58.2	879-930 bp	1.0	81.3
rpl20-rps12_p5	TTGTTGAGTAGCCGTTCGAG	CGCCATGCCAACCATTAAAC	50.0	50.0	57.9	58.4	958-1009 bp	1.0	81.2