Hotspot

petN-psbM

LSC · 29509-30329 · 821 bp

Back to Calypso

Pi 0.0341

MarkerSeek score 82.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 26
pi: 0.0341
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AATAAGTCTCGCTTGGGCTG	CTTGCGCTTATTGCTACTGC	50.0	50.0	58.0	57.9	985-993 bp	1.0	85.1
petN-psbM_p2	GGGACATCATTCGCATGGAT	CTTGCGCTTATTGCTACTGC	50.0	50.0	58.1	57.9	1007-1015 bp	1.0	84.5
petN-psbM_p3	AATAAGTCTCGCTTGGGCTG	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.0	58.3	924-932 bp	1.0	84.0
petN-psbM_p4	AATAAGTCTCGCTTGGGCTG	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.0	58.3	976-984 bp	1.0	83.8
petN-psbM_p5	GGGACATCATTCGCATGGAT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.1	58.3	946-954 bp	1.0	83.4