Hotspot

trnR-UCU-atpA

LSC · 10669-10849 · 181 bp

Back to Calypso

Pi 0.0237

MarkerSeek score 68.10

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 4
pi: 0.0237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGGGCGTCCATTGTCTAATG	CCGAAGAAGCTGAAACCCTT	50.0	50.0	57.7	58.1	322-333 bp	1.0	80.4
trnR-UCU-atpA_p2	AGGGCGTCCATTGTCTAATG	TCACCGAAGAAGCTGAAACC	50.0	50.0	57.7	58.1	325-336 bp	1.0	80.2
trnR-UCU-atpA_p3	GAAGGGCGTCCATTGTCTAA	CCGAAGAAGCTGAAACCCTT	50.0	50.0	57.6	58.1	324-335 bp	1.0	79.9
trnR-UCU-atpA_p4	GAAGGGCGTCCATTGTCTAA	TCACCGAAGAAGCTGAAACC	50.0	50.0	57.6	58.1	327-338 bp	1.0	79.7
trnR-UCU-atpA_p5	TAGGAATGAAGGGCGTCCAT	CCGAAGAAGCTGAAACCCTT	50.0	50.0	58.5	58.1	331-342 bp	1.0	79.0