Hotspot

trnS-GCU-trnG-GCC

LSC · 8546-9688 · 1143 bp

Back to Calypso

Pi 0.0174
MarkerSeek score 60.20
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1143
variable_sites
19
pi
0.0174
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 1173-1198 bp 1.0 52.8
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 1172-1197 bp 1.0 52.8
trnS-GCU-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA TTACCACTAAACTATACCCGC 50.0 42.9 58.0 54.9 1174-1199 bp 1.0 51.7
trnS-GCU-trnG-GCC_p4 AATCCGACGCTTTAGTCCAC TTACCACTAAACTATACCCGC 50.0 42.9 58.0 54.9 1173-1198 bp 1.0 51.7
trnS-GCU-trnG-GCC_p5 TAAAGTGATCAGGGGTCGGA TACCACTAAACTATACCCGC 50.0 45.0 57.8 54.0 1242-1267 bp 1.0 50.6