Hotspot

rps16-trnQ-UUG

LSC · 6111-7098 · 988 bp

Back to Calypso

Pi 0.0253

MarkerSeek score 73.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 988
variable_sites: 25
pi: 0.0253
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ATCCACAGCCAATCATGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1084-1127 bp	1.0	80.7
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1061-1104 bp	1.0	80.1
rps16-trnQ-UUG_p3	ATCCACAGCCAATCATGTCC	AGACCAAATCAAGATGGGGC	50.0	50.0	57.9	57.9	1146-1189 bp	1.0	80.0
rps16-trnQ-UUG_p4	CCACAGCCAATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1082-1125 bp	1.0	80.0
rps16-trnQ-UUG_p5	ATCCACAGCCAATCATGTCC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	57.9	57.8	1088-1131 bp	1.0	79.6