Hotspot

trnK-UUU-rps16

LSC · 4433-4971 · 539 bp

Back to Calypso

Pi 0.0261
MarkerSeek score 72.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
539
variable_sites
14
pi
0.0261
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 632-644 bp 1.0 67.3
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 631-643 bp 1.0 67.3
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 631-643 bp 1.0 66.5
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 630-642 bp 1.0 66.5
trnK-UUU-rps16_p5 AGGATCAGTCGCGGTCTTAT GTGCTCAACCCACAGGAAC 50.0 57.9 58.3 58.7 717-729 bp 1.0 66.0