Hotspot

ycf1

Genome · 106136-109282 · 3147 bp

Back to Calypogeia

Pi 0.0203
MarkerSeek score 52.80
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
Genome
length_bp
3147
variable_sites
64
pi
0.0203
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TGGATACTATGAGCATGTTTTTCA ACTGCTTGGGGGTTTCTAAC 33.3 50.0 56.4 57.7 260 bp 1.0 43.1
ycf1_p2 ATGGATACTATGAGCATGTTTTTCA ACTGCTTGGGGGTTTCTAAC 32.0 50.0 56.9 57.7 261 bp 1.0 43.1
ycf1_p3 TGGATACTATGAGCATGTTTTTCAT ACTGCTTGGGGGTTTCTAAC 32.0 50.0 56.9 57.7 260 bp 1.0 43.1
ycf1_p4 GGATACTATGAGCATGTTTTTCA ACTGCTTGGGGGTTTCTAAC 34.8 50.0 54.8 57.7 259 bp 1.0 43.1
ycf1_p5 TGGATACTATGAGCATGTTTTTC ACTGCTTGGGGGTTTCTAAC 34.8 50.0 54.8 57.7 260 bp 1.0 43.1