Hotspot

rpoC2

Genome · 12259-16464 · 4206 bp

Back to Calypogeia

Pi 0.0207

MarkerSeek score 50.80

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: Genome
length_bp: 4206
variable_sites: 87
pi: 0.0207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TGAAGGACGAACCAGCAAAA	GGTCCCTCCTCCAAAGTAGT	45.0	55.0	58.0	58.0	1138 bp	1.0	81.4
rpoC2_p2	TTGAAGGACGAACCAGCAAA	GGTCCCTCCTCCAAAGTAGT	45.0	55.0	58.0	58.0	1139 bp	1.0	81.4
rpoC2_p3	TGAAGGACGAACCAGCAAAA	TTGCTTCCTCCAAAACACGA	45.0	45.0	58.0	58.0	1215 bp	1.0	81.2
rpoC2_p4	TTGAAGGACGAACCAGCAAA	TTGCTTCCTCCAAAACACGA	45.0	45.0	58.0	58.0	1216 bp	1.0	81.2
rpoC2_p5	AGGACGAACCAGCAAAAACT	TTGCTTCCTCCAAAACACGA	45.0	45.0	57.9	58.0	1212 bp	1.0	81.2