Hotspot

petB

Genome · 73008-74217 · 1210 bp

Back to Calypogeia

Pi 0.0273

MarkerSeek score 61.10

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: Genome
length_bp: 1210
variable_sites: 33
pi: 0.0273
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	CCAAATTCTAGGTAGTTTAATCGTGT	GGAACTTCCTGGCCTATATATC	34.6	45.5	57.5	55.3	1293 bp	0.5	20.0
petB_p2	CCAAATTCTAGGTAGTTTAATCGTGT	GGAACTTCCTGGCCTATATA	34.6	45.0	57.5	53.3	1293 bp	0.5	20.0
petB_p3	TCCAAATTCTAGGTAGTTTAATCGTG	GGAACTTCCTGGCCTATATATC	34.6	45.5	57.3	55.3	1294 bp	0.5	20.0
petB_p4	TCCAAATTCTAGGTAGTTTAATCGTG	GGAACTTCCTGGCCTATATA	34.6	45.0	57.3	53.3	1294 bp	0.5	20.0
petB_p5	CCAAATTCTAGGTAGTTTAATCGTGT	GGAACTTCCTGGCCTATATAT	34.6	42.9	57.5	53.9	1293 bp	0.5	20.0