Hotspot

trnI-CAU-trnV-GAC

Genome · 82769-83253 · 485 bp

Back to Calypogeia

Pi 0.0507

MarkerSeek score 70.00

Primer available yes

Label trnI-CAU-trnV-GAC

Feature Metadata

label_name: trnI-CAU-trnV-GAC
feature_type: igs
region: Genome
length_bp: 485
variable_sites: 19
pi: 0.0507
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-trnV-GAC_p1	ACCATTCAGCCATGGATGC	CAGGCTCGAACTGATGACTT	52.6	50.0	58.2	57.6	456-566 bp	1.0	74.5
trnI-CAU-trnV-GAC_p2	ACCATTCAGCCATGGATGC	TCAGGCTCGAACTGATGACT	52.6	50.0	58.2	58.4	457-567 bp	1.0	73.9
trnI-CAU-trnV-GAC_p3	ACCATTCAGCCATGGATGC	GCCTCTTTCAATGGCGAAAA	52.6	45.0	58.2	57.3	512-621 bp	1.0	71.4
trnI-CAU-trnV-GAC_p4	ACCATTCAGCCATGGATGC	TGATGACTTTCACCACGTCA	52.6	45.0	58.2	57.1	445-555 bp	1.0	70.4
trnI-CAU-trnV-GAC_p5	ACCATTCAGCCATGGATGC	ACTGATGACTTTCACCACGT	52.6	45.0	58.2	57.1	447-557 bp	1.0	70.3