Hotspot

rps11-rpl36

Genome · 76894-76942 · 49 bp

Back to Calypogeia

Pi 0.122

MarkerSeek score 73.40

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: Genome
length_bp: 49
variable_sites: 6
pi: 0.122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CTGGACCTGGGCCATTAATC	GTGTAGTCACGGAATCGCTT	55.0	50.0	58.0	58.0	682 bp	1.0	81.6
rps11-rpl36_p2	CTGGACCTGGGCCATTAATC	CGAATATTGCCGGGGGATAG	55.0	55.0	58.0	58.0	578 bp	1.0	81.6
rps11-rpl36_p3	CTGGACCTGGGCCATTAATC	CGCTTCCGAATGCAATGTTT	55.0	45.0	58.0	58.0	667 bp	1.0	81.5
rps11-rpl36_p4	CTGGACCTGGGCCATTAATC	TTCCGAATGCAATGTTTCGC	55.0	45.0	58.0	58.0	664 bp	1.0	81.5
rps11-rpl36_p5	CTGGACCTGGGCCATTAATC	AATGTTTCGCGTTTGCTCAG	55.0	45.0	58.0	58.0	654 bp	1.0	81.4