Hotspot

rbcL-trnR-CCG

Genome · 59178-59247 · 70 bp

Back to Calypogeia

Pi 0.114

MarkerSeek score 72.80

Primer available yes

Label rbcL-trnR-CCG

Feature Metadata

label_name: rbcL-trnR-CCG
feature_type: igs
region: Genome
length_bp: 70
variable_sites: 8
pi: 0.114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-trnR-CCG_p1	TTGCTCGCGAAGGTAATGAA	GGATTCGAACCCTTGACCTC	45.0	55.0	57.9	58.0	252-253 bp	1.0	82.9
rbcL-trnR-CCG_p2	TGCCAGCCTCAACAGAAATT	GGATTCGAACCCTTGACCTC	45.0	55.0	58.0	58.0	402-403 bp	1.0	82.6
rbcL-trnR-CCG_p3	AATTGGAAGGGGAACGTGAC	GGATTCGAACCCTTGACCTC	50.0	55.0	58.1	58.0	561-562 bp	1.0	81.0
rbcL-trnR-CCG_p4	TTTGGCCCACTATTGTCGAG	GGATTCGAACCCTTGACCTC	50.0	55.0	57.9	58.0	725-726 bp	1.0	80.7
rbcL-trnR-CCG_p5	TATCTGGTGGGGATCACGTT	GGATTCGAACCCTTGACCTC	50.0	55.0	58.1	58.0	603 bp	0.5	58.8