Hotspot

psbA-trnH-GUG

Genome · 30110-30238 · 129 bp

Back to Calypogeia

Pi 0.0465

MarkerSeek score 70.40

Primer available yes

Label psbA-trnH-GUG

Feature Metadata

label_name: psbA-trnH-GUG
feature_type: igs
region: Genome
length_bp: 129
variable_sites: 6
pi: 0.0465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnH-GUG_p1	CAACCGTGCTAACCTTGGTA	CTGCCTTAATCCACTTGGCT	50.0	50.0	57.8	57.9	256 bp	1.0	79.4
psbA-trnH-GUG_p2	ATCAACCGTGCTAACCTTGG	CTGCCTTAATCCACTTGGCT	50.0	50.0	58.2	57.9	258 bp	1.0	79.4
psbA-trnH-GUG_p3	CAACCGTGCTAACCTTGGTA	GATCCACAATCCACTGCCTT	50.0	50.0	57.8	57.9	269 bp	1.0	79.3
psbA-trnH-GUG_p4	ATCAACCGTGCTAACCTTGG	GATCCACAATCCACTGCCTT	50.0	50.0	58.2	57.9	271 bp	1.0	79.3
psbA-trnH-GUG_p5	CAACCGTGCTAACCTTGGTA	ACTGCCTTAATCCACTTGGC	50.0	50.0	57.8	58.2	257 bp	1.0	79.2