Hotspot

ycf1

SSC · 125088-130574 · 5487 bp

Back to Calycanthus

Pi 0.00364

MarkerSeek score 42.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5487
variable_sites: 30
pi: 0.00364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGGACAAGGAAGATAGATCCC	GGTTCATACGTAAATGGCGC	52.4	50.0	56.9	57.6	276 bp	1.0	61.7
ycf1_p2	GGGACAAGGAAGATAGATCCC	TATTCCCAGGGTCAATGCAC	52.4	50.0	56.9	57.6	197 bp	1.0	61.7
ycf1_p3	GGGACAAGGAAGATAGATCCC	CCAGGGTCAATGCACAACTT	52.4	50.0	56.9	58.7	192 bp	1.0	59.8
ycf1_p4	GGGACAAGGAAGATAGATCCCA	GGTTCATACGTAAATGGCGC	50.0	50.0	58.7	57.6	276 bp	1.0	56.7
ycf1_p5	TGGGACAAGGAAGATAGATCCC	GGTTCATACGTAAATGGCGC	50.0	50.0	58.7	57.6	277 bp	1.0	56.7