Hotspot

petD

LSC · 78089-79291 · 1203 bp

Back to Calycanthus

Pi 0.00277

MarkerSeek score 52.30

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1203
variable_sites: 5
pi: 0.00277
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TCTCCGAAGAGAAGATGGAT	TGAATCTTTAGCACGATCCGT	45.0	42.9	55.0	57.5	1335 bp	1.0	44.5
petD_p2	TCTCCGAAGAGAAGATGGATT	TGAATCTTTAGCACGATCCGT	42.9	42.9	55.9	57.5	1335 bp	1.0	43.2
petD_p3	TCTCCGAAGAGAAGATGGAT	GGGAGAATTCACTTTGAAGCC	45.0	47.6	55.0	57.1	1271 bp	1.0	41.9
petD_p4	TCTCCGAAGAGAAGATGGATT	GGGAGAATTCACTTTGAAGCC	42.9	47.6	55.9	57.1	1271 bp	1.0	40.5
petD_p5	TCTCCGAAGAGAAGATGGAT	ACTTTGAAGCCACTATTCCCT	45.0	42.9	55.0	56.9	1261 bp	1.0	40.3