Hotspot

psaC-ndhE

SSC · 118617-118890 · 274 bp

Back to Calycanthus

Pi 0.00244

MarkerSeek score 54.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 274
variable_sites: 1
pi: 0.00244
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	CTATTGGGCCGGCTATTGTT	50.0	50.0	58.1	58.0	458-459 bp	1.0	79.2
psaC-ndhE_p2	CAATACATCTGTGGGGCAGG	CTATTGGGCCGGCTATTGTT	55.0	50.0	58.3	58.0	434-435 bp	1.0	77.7
psaC-ndhE_p3	CCAATACATCTGTGGGGCAG	CTATTGGGCCGGCTATTGTT	55.0	50.0	58.3	58.0	435-436 bp	1.0	77.7
psaC-ndhE_p4	CAGGCTCGTACACATTGAGT	CTATTGGGCCGGCTATTGTT	50.0	50.0	57.6	58.0	418-419 bp	1.0	77.3
psaC-ndhE_p5	AGCTTTACATCCGTCCCAAG	CTATTGGGCCGGCTATTGTT	50.0	50.0	57.6	58.0	464-465 bp	1.0	77.0