Hotspot

trnR(acg)-trnN(guu)

IRb · 109220-109822 · 603 bp

Back to Calycanthus

Pi 0.00553

MarkerSeek score 62.30

Primer available yes

Label trnR(acg)-trnN(guu)

Feature Metadata

label_name: trnR(acg)-trnN(guu)
feature_type: igs
region: IRb
length_bp: 603
variable_sites: 5
pi: 0.00553
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR(acg)-trnN(guu)_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	744-748 bp	1.0	78.8
trnR(acg)-trnN(guu)_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	781-785 bp	1.0	78.2
trnR(acg)-trnN(guu)_p3	CAGAGGATTAGAGCACGTGG	CGGTTGGCTGTTAACTGACT	55.0	50.0	57.8	58.1	714-718 bp	1.0	77.9
trnR(acg)-trnN(guu)_p4	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	708-712 bp	1.0	77.6
trnR(acg)-trnN(guu)_p5	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	695-699 bp	1.0	77.6