Hotspot

rpl20-rps12

LSC · 70576-71345 · 770 bp

Back to Calycanthus

Pi 0.00346

MarkerSeek score 61.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 770
variable_sites: 4
pi: 0.00346
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	AATTTTTGTTCGACGTCGCC	GAAATCCCCTGCTCTTCGAG	45.0	55.0	58.0	58.1	879-881 bp	1.0	79.7
rpl20-rps12_p2	CTTGAATGAGCCCCTCGAAA	GAAATCCCCTGCTCTTCGAG	50.0	55.0	57.9	58.1	917-919 bp	1.0	79.0
rpl20-rps12_p3	TTGAATGAGCCCCTCGAAAG	GAAATCCCCTGCTCTTCGAG	50.0	55.0	57.9	58.1	916-918 bp	1.0	79.0
rpl20-rps12_p4	AATTTTTGTTCGACGTCGCC	ACAGCCAATCCGAAATGTCA	45.0	45.0	58.0	57.8	900-902 bp	1.0	78.5
rpl20-rps12_p5	AATTTTTGTTCGACGTCGCC	ACAAGACAGCCAATCCGAAA	45.0	45.0	58.0	57.7	905-907 bp	1.0	78.0