Hotspot

trnS(uga)-psbZ

LSC · 36839-37050 · 212 bp

Back to Calycanthus

Pi 0
MarkerSeek score 23.00
Primer available yes
Label trnS(uga)-psbZ

Feature Metadata

label_name
trnS(uga)-psbZ
feature_type
igs
region
LSC
length_bp
212
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
23.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS(uga)-psbZ_p1 CAAGACCGGAGCTATCAACC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.1 57.0 281 bp 1.0 71.6
trnS(uga)-psbZ_p2 CTATCAACCACTCGGCCATC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.1 57.0 270 bp 1.0 71.0
trnS(uga)-psbZ_p3 CGAGCAAAAGGAGAGAGAGG TGCAAAAACAGCCAATTGGA 55.0 40.0 57.7 57.0 346 bp 1.0 69.7
trnS(uga)-psbZ_p4 AGCAAAAGGAGAGAGAGGGA TGCAAAAACAGCCAATTGGA 50.0 40.0 57.7 57.0 344 bp 1.0 69.4
trnS(uga)-psbZ_p5 CAAGACCGGAGCTATCAACC TGCAAAAACAGCCAATTGGAA 55.0 38.1 58.1 57.7 281 bp 1.0 69.4