Hotspot

psbC-trnS(uga)

LSC · 36526-36745 · 220 bp

Back to Calycanthus

Pi 0.0242

MarkerSeek score 68.00

Primer available yes

Label psbC-trnS(uga)

Feature Metadata

label_name: psbC-trnS(uga)
feature_type: igs
region: LSC
length_bp: 220
variable_sites: 8
pi: 0.0242
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS(uga)_p1	TCCTATTCGTGGGCCATTTG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.9	58.1	413 bp	1.0	80.7
psbC-trnS(uga)_p2	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	327 bp	1.0	80.2
psbC-trnS(uga)_p3	TCCTATTCGTGGGCCATTTG	GATGGCCGAGTGGTTGATAG	50.0	55.0	57.9	58.1	424 bp	1.0	80.1
psbC-trnS(uga)_p4	TCCTATTCGTGGGCCATTTG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.9	58.2	359 bp	1.0	79.6
psbC-trnS(uga)_p5	CCTGTTCTTTCCATGACCCC	GATGGCCGAGTGGTTGATAG	55.0	55.0	58.2	58.1	338 bp	1.0	79.6