Hotspot

atpH-atpI

LSC · 14140-15238 · 1099 bp

Back to Calycanthus

Pi 0.0303

MarkerSeek score 53.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1099
variable_sites: 49
pi: 0.0303
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCAGAAATCAGTGGATTCAT	TTTTTGCAACTTTAGCCGCG	40.0	45.0	53.6	58.5	1158-1175 bp	1.0	45.8
atpH-atpI_p2	GCAGAAATCAGTGGATTCAT	CAAGCGGGATTCAAGCTCTT	40.0	50.0	53.6	58.5	1179-1196 bp	1.0	45.5
atpH-atpI_p3	GCAGAAATCAGTGGATTCAT	GGTGAATCCATGGAAGGTCA	40.0	50.0	53.6	57.2	1129-1146 bp	1.0	45.0
atpH-atpI_p4	GCAGAAATCAGTGGATTCAT	TTAGCCGCGGCCTATATAGG	40.0	55.0	53.6	58.9	1147-1164 bp	1.0	44.9
atpH-atpI_p5	GCAGAAATCAGTGGATTCAT	TTTAGCCGCGGCCTATATAG	40.0	50.0	53.6	57.0	1148-1165 bp	1.0	44.9