Hotspot

atpA-atpF

LSC · 12096-12162 · 67 bp

Back to Calycanthus

Pi 0

MarkerSeek score 55.60

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 67
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGTCGCCTACTTGAAGTAC	CAGCGCTAATATTGGCATGC	55.0	50.0	58.1	58.0	227-229 bp	1.0	79.7
atpA-atpF_p2	CCGTCGCCTACTTGAAGTAC	AGCGCTAATATTGGCATGCT	55.0	45.0	58.1	58.1	226-228 bp	1.0	79.2
atpA-atpF_p3	CCGTCGCCTACTTGAAGTAC	TAATATTGGCATGCTCGGGG	55.0	50.0	58.1	57.8	221-223 bp	1.0	78.4
atpA-atpF_p4	CCGTCGCCTACTTGAAGTAC	CTCGGGGCCATGAAAGAAAT	55.0	50.0	58.1	58.2	208-210 bp	1.0	78.2
atpA-atpF_p5	CCGTCGCCTACTTGAAGTAC	GCGCTAATATTGGCATGCTC	55.0	50.0	58.1	57.7	225-227 bp	1.0	77.9