Hotspot

trnK(uuu)-rps16

LSC · 4208-5010 · 803 bp

Back to Calycanthus

Pi 0.00416
MarkerSeek score 61.60
Primer available yes
Label trnK(uuu)-rps16

Feature Metadata

label_name
trnK(uuu)-rps16
feature_type
igs
region
LSC
length_bp
803
variable_sites
5
pi
0.00416
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK(uuu)-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAGGGCGCTCAACCTAC 50.0 52.6 58.2 58.1 932-934 bp 1.0 70.8
trnK(uuu)-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAGGGCGCTCAACCTAC 50.0 52.6 58.2 58.1 931-933 bp 1.0 70.8
trnK(uuu)-rps16_p3 CCGCACTTAAAAGCCGAGTA AAAAGGGCGCTCAACCTAC 50.0 52.6 58.3 58.1 941-943 bp 1.0 69.9
trnK(uuu)-rps16_p4 AAAGCCGAGTACTCTACCGT GAAAAGGGCGCTCAACCTAC 50.0 55.0 58.2 59.2 933-935 bp 1.0 69.7
trnK(uuu)-rps16_p5 AAGCCGAGTACTCTACCGTT GAAAAGGGCGCTCAACCTAC 50.0 55.0 58.2 59.2 932-934 bp 1.0 69.7