Hotspot

ycf1

SSC · 135044-141340 · 6297 bp

Back to Calotropis

Pi 0.00128

MarkerSeek score 54.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 6297
variable_sites: 8
pi: 0.00128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTCCAATAGCGGGTCTTTC	GGAATCGGAATTGGAATCGGA	50.0	47.6	57.3	58.2	308 bp	1.0	69.7
ycf1_p2	GTCCAATAGCGGGTCTTTCT	GGAATCGGAATTGGAATCGGA	50.0	47.6	57.3	58.2	307 bp	1.0	69.7
ycf1_p3	AGTCCAATAGCGGGTCTTTC	GGAATCGGAATTGGAATCGG	50.0	50.0	57.3	56.6	308 bp	1.0	68.2
ycf1_p4	GTCCAATAGCGGGTCTTTCT	GGAATCGGAATTGGAATCGG	50.0	50.0	57.3	56.6	307 bp	1.0	68.2
ycf1_p5	GTCCAATAGCGGGTCTTTCT	CGGAATTGGAATCGGAATTGG	50.0	47.6	57.3	57.6	302 bp	1.0	68.2