Hotspot

petB

LSC · 84743-86177 · 1435 bp

Back to Calotropis

Pi 0.00139

MarkerSeek score 59.60

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1435
variable_sites: 2
pi: 0.00139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TCATTTAGAGCCCGGGTTTC	TCCTTCCCCAAGTGATAAATGA	50.0	40.9	57.6	56.8	1572 bp	1.0	51.1
petB_p2	GGGAGTTCAATCGCGGAATT	TCCTTCCCCAAGTGATAAATGA	50.0	40.9	58.6	56.8	1531 bp	1.0	49.4
petB_p3	GGGTTTCGAGCCCATTCTAT	TCCTTCCCCAAGTGATAAATGA	50.0	40.9	57.4	56.8	1559 bp	1.0	49.2
petB_p4	TCATTTAGAGCCCGGGTTTC	TTCCTTCCCCAAGTGATAAATGA	50.0	39.1	57.6	57.5	1573 bp	1.0	48.6
petB_p5	ATTTTGGGAGTTCAATCGCG	TCCTTCCCCAAGTGATAAATGA	45.0	40.9	57.1	56.8	1536 bp	1.0	47.0