Hotspot

ccsA-ndhD

SSC · 126514-126753 · 240 bp

Back to Calotropis

Pi 0.025

MarkerSeek score 66.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 240
variable_sites: 6
pi: 0.025
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTCGGCAATTGTAGCGTGTA	ACCCGGATTTCGTTCTTTCA	45.0	45.0	57.9	57.4	426 bp	1.0	76.0
ccsA-ndhD_p2	TTCGGCAATTGTAGCGTGTA	TCGGTTGACAAGGTTGAAGT	45.0	45.0	57.9	57.3	403 bp	1.0	74.9
ccsA-ndhD_p3	TCGGCAATTGTAGCGTGTAT	ACCCGGATTTCGTTCTTTCA	45.0	45.0	57.7	57.4	425 bp	1.0	74.3
ccsA-ndhD_p4	TTCGGCAATTGTAGCGTGTA	CCCGGATTTCGTTCTTTCAC	45.0	50.0	57.9	57.1	425 bp	1.0	73.3
ccsA-ndhD_p5	TTCGGCAATTGTAGCGTGTA	TGGTATGTACCCGGATTTCG	45.0	50.0	57.9	57.1	434 bp	1.0	73.3