Hotspot

ndhF-rpl32

SSC · 123454-124289 · 836 bp

Back to Calotropis

Pi 0.00123

MarkerSeek score 62.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 836
variable_sites: 1
pi: 0.00123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGAAGGGAAAGTGGGATGA	AGCTTTTAATGCTGCCCGAT	50.0	45.0	57.9	58.2	964-986 bp	1.0	79.6
ndhF-rpl32_p2	GGTATGAGCCACCCGTATTG	AGCTTTTAATGCTGCCCGAT	55.0	45.0	58.1	58.2	942-964 bp	1.0	79.1
ndhF-rpl32_p3	AGGAAGGGAAAGTGGGATGA	TAATGCTGCCCGATATCCCT	50.0	50.0	57.9	58.3	958-980 bp	1.0	78.7
ndhF-rpl32_p4	AGGAAGGGAAAGTGGGATGA	AAAGCTTTTAATGCTGCCCG	50.0	45.0	57.9	57.6	966-988 bp	1.0	78.5
ndhF-rpl32_p5	GGTATGAGCCACCCGTATTG	TAATGCTGCCCGATATCCCT	55.0	50.0	58.1	58.3	936-958 bp	1.0	78.2