Hotspot

rpl20-rps12

LSC · 78529-79462 · 934 bp

Back to Calotropis

Pi 0

MarkerSeek score 59.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 934
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GTAAGTCTTGAATGGGCCCC	CTCAACGTCGAGGAACATGT	55.0	50.0	58.2	57.9	1050-1061 bp	1.0	78.4
rpl20-rps12_p2	CCCCTCGAAAGCTTAATGCA	CTCAACGTCGAGGAACATGT	50.0	50.0	58.2	57.9	1034-1045 bp	1.0	78.3
rpl20-rps12_p3	TAAGTCTTGAATGGGCCCCT	CTCAACGTCGAGGAACATGT	50.0	50.0	58.3	57.9	1049-1060 bp	1.0	77.8
rpl20-rps12_p4	GTAAGTCTTGAATGGGCCCC	ACAGCCAATCAGAAATCGCA	55.0	45.0	58.2	58.2	1097-1108 bp	1.0	77.5
rpl20-rps12_p5	GTAAGTCTTGAATGGGCCCC	CAGCCAATCAGAAATCGCAC	55.0	50.0	58.2	57.8	1096-1107 bp	1.0	77.4