Hotspot

petA-psbJ

LSC · 72788-73389 · 602 bp

Back to Calotropis

Pi 0.00166

MarkerSeek score 59.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 602
variable_sites: 1
pi: 0.00166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ATTCTGGATTGGGTTCGTCC	50.0	50.0	58.0	57.6	747 bp	1.0	76.4
petA-psbJ_p2	ATTACGTGTCCAAGGCCTTG	ATTCTGGATTGGGTTCGTCC	50.0	50.0	58.2	57.6	743 bp	1.0	75.3
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	ATTCTGGATTGGGTTCGTCC	50.0	50.0	58.2	57.6	744 bp	1.0	75.3
petA-psbJ_p4	CGTGTCCAAGGCCTTGTATT	ATTCTGGATTGGGTTCGTCC	50.0	50.0	58.2	57.6	739 bp	1.0	75.3
petA-psbJ_p5	TACGTGTCCAAGGCCTTGTA	ATTCTGGATTGGGTTCGTCC	50.0	50.0	58.7	57.6	741 bp	1.0	71.5