Hotspot

ycf4-cemA

LSC · 70222-70932 · 711 bp

Back to Calotropis

Pi 0.00281

MarkerSeek score 60.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 711
variable_sites: 2
pi: 0.00281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGAGAGAAAGCCACCAAGG	45.0	55.0	58.1	57.9	815 bp	1.0	78.6
ycf4-cemA_p2	AAAAAGCTGCTGAATTGGCC	GAGAGAGAAAGCCACCAAGG	45.0	55.0	57.8	57.9	829 bp	1.0	77.9
ycf4-cemA_p3	AAAGCTGCTGAATTGGCCTA	GAGAGAGAAAGCCACCAAGG	45.0	55.0	57.8	57.9	827 bp	1.0	77.6
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	AGAGAGAAAGCCACCAAGGA	45.0	50.0	58.1	58.3	814 bp	1.0	77.3
ycf4-cemA_p5	TTGGCCTATTTCTTGCGTGT	CCGGCATTCCACGAATTAGT	45.0	50.0	58.1	58.3	863 bp	1.0	76.7