Hotspot

rbcL-accD

LSC · 63756-64487 · 732 bp

Back to Calotropis

Pi 0.00137

MarkerSeek score 45.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 732
variable_sites: 1
pi: 0.00137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	ACTACAGTTCGGTGGAGGAA	CATAAACCACCGTTTTCGCC	50.0	50.0	58.0	58.0	991 bp	1.0	79.9
rbcL-accD_p2	TGCAATGCACGCAGTTATTG	CATAAACCACCGTTTTCGCC	45.0	50.0	58.0	58.0	1303 bp	1.0	79.7
rbcL-accD_p3	GAAACCGGCGAAATCAAAGG	CATAAACCACCGTTTTCGCC	50.0	50.0	58.0	58.0	1497 bp	1.0	79.7
rbcL-accD_p4	ACTACAGTTCGGTGGAGGAA	ACCATCGTTATCTTGCGCAT	50.0	45.0	58.0	58.0	1444 bp	1.0	79.7
rbcL-accD_p5	TGCAATGCACGCAGTTATTG	ACCATCGTTATCTTGCGCAT	45.0	45.0	58.0	58.0	1756 bp	1.0	79.6