Hotspot

ndhC-trnV-UAC

LSC · 57223-58543 · 1321 bp

Back to Calotropis

Pi 0.000764
MarkerSeek score 60.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1321
variable_sites
1
pi
0.000764
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGTTAATCGGGGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1455-1466 bp 1.0 78.1
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1441-1452 bp 1.0 78.0
ndhC-trnV-UAC_p3 GTCCTTTGTTAATCGGGGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1454-1465 bp 1.0 75.4
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1440-1451 bp 1.0 75.3
ndhC-trnV-UAC_p5 TGGTCCTTTGTTAATCGGGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.5 57.8 1457-1468 bp 1.0 75.1