Hotspot

atpA-atpF

LSC · 11941-12015 · 75 bp

Back to Calotropis

Pi 0.0133

MarkerSeek score 60.20

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 75
variable_sites: 1
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ATGCCATCGCCTACTTGAAG	TTGGCAGGTTAGGGTCAATG	50.0	50.0	58.0	57.8	229-239 bp	1.0	78.3
atpA-atpF_p2	ATGCCATCGCCTACTTGAAG	TACGTGGAGCCTTAGGAACT	50.0	50.0	58.0	57.8	301-311 bp	1.0	78.1
atpA-atpF_p3	ATCGCCTACTTGAAGTACGC	TTGGCAGGTTAGGGTCAATG	50.0	50.0	57.8	57.8	224-234 bp	1.0	77.0
atpA-atpF_p4	ATCGCCTACTTGAAGTACGC	TACGTGGAGCCTTAGGAACT	50.0	50.0	57.8	57.8	296-306 bp	1.0	76.8
atpA-atpF_p5	CAATGCCATCGCCTACTTGA	TTGGCAGGTTAGGGTCAATG	50.0	50.0	58.3	57.8	231-241 bp	1.0	76.0