Hotspot

trnK-UUU-rps16

LSC · 4421-5194 · 774 bp

Back to Calotropis

Pi 0.00129
MarkerSeek score 59.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
774
variable_sites
1
pi
0.00129
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 GTCGTGGTCTTCCAAACCTT GGGTGGGGGTTTTTAAGGAA 50.0 50.0 58.0 57.6 940 bp 1.0 76.6
trnK-UUU-rps16_p2 GTCGTGGTCTTCCAAACCTT AAGGGTGGGGGTTTTTAAGG 50.0 50.0 58.0 57.6 942 bp 1.0 76.5
trnK-UUU-rps16_p3 CCGATGGTATGGACGAATCC GGGTGGGGGTTTTTAAGGAA 55.0 50.0 57.9 57.6 919 bp 1.0 76.2
trnK-UUU-rps16_p4 GTCGTGGTCTTCCAAACCTT AGGGTGGGGGTTTTTAAGGA 50.0 50.0 58.0 58.5 941 bp 1.0 76.2
trnK-UUU-rps16_p5 CCGATGGTATGGACGAATCC AAGGGTGGGGGTTTTTAAGG 55.0 50.0 57.9 57.6 921 bp 1.0 76.2