Hotspot

trnN-GUU-trnR-ACG

IRa · 136143-136775 · 633 bp

Back to Calophyllum

Pi 0.0079

MarkerSeek score 63.70

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 633
variable_sites: 7
pi: 0.0079
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TCCTCCCATTGTTCCTCAGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.0	57.8	779 bp	1.0	78.6
trnN-GUU-trnR-ACG_p2	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	738 bp	1.0	77.8
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	725 bp	1.0	77.8
trnN-GUU-trnR-ACG_p4	GGGTTGAGAACGGGAATTGA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	815 bp	1.0	77.4
trnN-GUU-trnR-ACG_p5	CTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.8	57.8	726 bp	1.0	77.4