Hotspot

psaJ-rpl33

LSC · 71312-71840 · 529 bp

Back to Calophyllum

Pi 0.0202

MarkerSeek score 61.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 529
variable_sites: 16
pi: 0.0202
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GTTCGGGTCTTTAGCAGGTC	TCGAACATCTTTCCCCTTGG	55.0	50.0	58.3	57.5	624-635 bp	1.0	77.5
psaJ-rpl33_p2	TTCGGGTCTTTAGCAGGTCT	TCGAACATCTTTCCCCTTGG	50.0	50.0	58.4	57.5	623-634 bp	1.0	76.9
psaJ-rpl33_p3	ATGGTTCGGGTCTTTAGCAG	TCGAACATCTTTCCCCTTGG	50.0	50.0	57.6	57.5	627-638 bp	1.0	76.5
psaJ-rpl33_p4	TCGGGTCTTTAGCAGGTCTA	TCGAACATCTTTCCCCTTGG	50.0	50.0	57.5	57.5	622-633 bp	1.0	75.6
psaJ-rpl33_p5	GTTCGGGTCTTTAGCAGGTC	GAACATCTTTCCCCTTGGCC	55.0	55.0	58.3	58.8	622-633 bp	1.0	75.0